Did anybody worked with EphA family protein expression? MW 130kDa . I am not getting any expression. Do you use different lysis buffer or RIPA?

I am working on EphA2, pEpA3 expression with different cell types (MD-MBA231, MOLT4) for the expression of EphA family proteins, They are of high molecular weight 130 kDa. I use RIPA buffer and...

01 December 2020 8,610 1 View

What statistical test should I use if I want to compare values of temperature taken at aural, forehead and inner wrist ?

I basically want to see if there is a difference between these sites. Especially between the forehead and inner wrist as many patients are not very happy with temperature reading taken at the...

05 October 2020 3,151 12 View

Need weather and metrological data for forecasting or predicting the agriculture in Saudi Arabia?

Need weather and metrological data for forecasting or predicting agriculture in Saudi Arabia? This is for the purpose of one of the project by my fellow researchers. Kindly share the same.

11 August 2020 8,163 4 View

What are the high impact journals available in sustainability and sustainable development domain?

What are the high impact journals available in sustainability and sustainable development domain?

24 June 2020 3,979 11 View

What are the COVID-19 papers retracted from journals?is it clinical, treatment, diagnosis or AI based prediction? Why?

What are the COVID-19 papers retracted from journals? is it related to clinical, treatment, diagnosis or AI-based prediction? Why

07 June 2020 7,301 3 View

Hydroxychlorine was proposed as drug for corona viral disease (COVID-19). Is it good for treatment?

It might induce immunosuppression.

03 April 2020 3,740 4 View

Quantitative & Qualitative perceptive. (mix mode)?

Dear All, My research topic is socio-economic impact of employment of Filipinos Maids in Singapore. My research will be both Quantitative & Qualitative perceptive. (mix mode) My question:...

19 October 2019 5,959 8 View

How can we determine the sample size from an unknown population?

Please provide example too, im new ...

09 April 2019 5,724 4 View

Transformed colonies with GFP showed Faded Fluorescence ?

Transformed Yeast colonies with a Plasmid having GFP, showed faded Fluorescence. It was light yellowish in colour rather than the usual Green Colour . What could be the reason ? The Microscope was...

28 December 2018 7,516 4 View

Doubt on microbiological testing ?

I am conducting microbiological testing on QC samples recently. I did a test to get Total bacterial count (TBC) on a sample. And I used pour plate method. I am encountering some errors recently....

15 August 2018 6,673 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View