I tried, but every time after xylene and paraffin treatment, when I open the cassette, I was unable to detect the aorta sample. As it becomes colorless and shrunken so I cannot detect/find that, resulting in loss of sample. Any suggestions to improve procedure/method.
Alexander Oberhuber
Years ago I "fixed" the aorta (or other vessels) perpendicular in a liver segment of the mouse:
1) harvest the biggest liver segment, eg. the lobus lateralis sinister
2) make a incision nearly the half of the diameter
3) put the aorta perpendicular into the liver
4) fix the incision with a simple stitch
So you can always find the aorta and after embedding it in paraffin it is much easier to cut your slices perpendicular to the lumen und you really get "rings"
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