@Felix Toka: that´s a anti-CCR5 antibody.. I wouldn´t recommend the BD antibody, better use the one from ebioscience

http://www.ebioscience.com/mouse-cd195-antibody-pe-hm-ccr5.htm

(I had positive stainings within CCR5ko mice, but not with the ebioscience Ab)

@Wei Zhang

like Thomas Haupt said: try to find a mono-/or polyclonal Ab on the market without fluorochrome and then to label it with a secondary one or with a labeling kit, e.g. zenon (http://www.lifetechnologies.com/de/de/home/brands/molecular-probes/key-molecular-probes-products/zenon-labeling-technology.html)

Good luck

Have you tried the BD Biosciences 559923 PE conjugate?

Hi Wei,

A brief search - that you have probably done much more thoroughly - reveals that a fluorochrome-conjugated anti-CCL5 for mice probably isn't on the market.

However, you can just choose a primary anti-CCL5 antibody and add a secondary antibody that is fluorochrome-conjugated (e.g. http://www.ebioscience.com/rabbit-igg-isotype-control-pe-polyclonal.htm if your primary antibody is from rabbit).

Try to find a primary antibody that is validated for flow cytometry. This makes titration a lot easier since they usually suggest a starting concentration. The secondary AB should also be titrated, but the company should provide a suggestion for the concentration.

Good luck.

These days the world is full of search engines. Trying any of these specific antibody search engines...

http://www.citeab.com/

http://www.abcam.com/

http://www.tebu-bio.com/kdb/techinfo.html#search_live

http://www.antibodyresource.com/findantibody.html

...gives results for your target - in order of my decreasing preference.

CiteAb looks very good, giving you information on actual publications where the antibodies were used - thus a good source of useful information on how the antibodies were used successfully. In addition, CiteAb gives you a list of supplier.

Good luck!

@Felix Toka: that´s a anti-CCR5 antibody.. I wouldn´t recommend the BD antibody, better use the one from ebioscience

http://www.ebioscience.com/mouse-cd195-antibody-pe-hm-ccr5.htm

(I had positive stainings within CCR5ko mice, but not with the ebioscience Ab)

@Wei Zhang

like Thomas Haupt said: try to find a mono-/or polyclonal Ab on the market without fluorochrome and then to label it with a secondary one or with a labeling kit, e.g. zenon (http://www.lifetechnologies.com/de/de/home/brands/molecular-probes/key-molecular-probes-products/zenon-labeling-technology.html)

Good luck

Actually, I meant that he could just incubate with the primary AB, wash, and then incubate with the secondary AB.

Anyway, I see you have found a link for a nice PE-conjugated anti-CCL5 AB, so no need to make it difficult. I just didn't bother looking much at Google, I presumed he did that already.

I am sorry for the wrong answer.

@Jochen, thanks for catching that!

I'm sorry I did not work with mouse RANTES antibody

You must ask Mark Dahlquist of R&D Systems or ask William Hunter of Mesoscale discovery, but their Abs are all obtained from R&D or other sources.

Krishna

We use a rabbit anti-mouse pAb from eBiosciences, but they stopped selling it. Try their new mouse-anti-mouse RANTES. If it does not work for you, we may be able to send you some pAb.

Klaus

Hi Ley,

Thank you very much for your good advice and generous help.I will try new mouse-anti-mouse RANTES from eBiosciences first.