i am growing a bacteria in TB medium and i suspect presence of one coloured compound to be an indole's derivative. is there any recommended method to extract this compound in order to obtain better absorption scanning on spectrophotometry ?
I don't know how much sample amount. So, you may employ chromatographic separation if the amount is small. Or you may take your bacteria supernatant, then use organic/aq separation --solvents like methanol, petroleum ether (organic phase)/H2O (aq phase). Also, vacuum evaporator can be used to preconcentrate the concentration in case your concentration is too low. And the color will be developed via the p-dimethylaminocinnamaldehyde reaction.