We have had some experience with 5-MTHF measurements in rice (endosperm), arabidopsis and potato (tubers) (see papers).
De Brouwer, V., Storozhenko, S., Stove, C. P., Van Daele, J., Van Der Straeten, D., & Lambert, W. E. (2010). Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) for the sensitive determination of folates in rice. Journal of Chromatography B, 878(3-4), 509-513.
Navarrete, O., Van Daele, J., Stove, C., Lambert, W., Van Der Straeten, D., & Storozhenko, S. (2012). A folate independent role for cytosolic HPPK/DHPS upon stress in Arabidopsis thaliana. Phytochemistry, 73, 23-33.
De Lepeleire, J., Strobbe, S., Verstraete, J., Blancquaert, D., Ambach, L., Visser, R. G., ... & Van Der Straeten, D. (2018). Folate Biofortification of Potato by Tuber-Specific Expression of Four Folate Biosynthesis Genes. Molecular plant, 11(1), 175-188
Hope this helps
We have had some experience with 5-MTHF measurements in rice (endosperm), arabidopsis and potato (tubers) (see papers).
De Brouwer, V., Storozhenko, S., Stove, C. P., Van Daele, J., Van Der Straeten, D., & Lambert, W. E. (2010). Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) for the sensitive determination of folates in rice. Journal of Chromatography B, 878(3-4), 509-513.
Navarrete, O., Van Daele, J., Stove, C., Lambert, W., Van Der Straeten, D., & Storozhenko, S. (2012). A folate independent role for cytosolic HPPK/DHPS upon stress in Arabidopsis thaliana. Phytochemistry, 73, 23-33.
De Lepeleire, J., Strobbe, S., Verstraete, J., Blancquaert, D., Ambach, L., Visser, R. G., ... & Van Der Straeten, D. (2018). Folate Biofortification of Potato by Tuber-Specific Expression of Four Folate Biosynthesis Genes. Molecular plant, 11(1), 175-188
Hope this helps
addition journal:
Article Application of an HPLC assay for the determination of folate...
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