Can anybody suugest some primary cells or cell lines for IBD/Cronh's diseases? What is the common protocol to induce inflammation in the healthy cells in vitro? Is there any diseased/transgenic cell lines?
Geoffrey Ian Sandle
Hi
Cannot see how you could replicate what happens in colonic epithelium in IBD using cells lines. Maintaining normal human colonic epithelial cells in culture is very difficult, and doing the same with inflamed cells from IBD patients likely to be even harder. The dextran sulphate model of acute colitis in rats produces the same histological features and ion transporter/channel abnormalities as seen in human ulcerative colitis. Hope that helps.
Regards
Professor Geoffrey Sandle
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