Any suggestion for human VEGF primer for Realtime PCR? Struggling with dimers!

Farzad Seyed Forootan @Farzad_Seyed_Forootan

01 January 2017 1 9K Report

If you have any human VEGF primer sequence, please let me know.

I used several software for designing but struggle with dimer formation

ALEX CHUN CHEUNG

forward, 5′-ctacctccaccatgccaagt-3′; and

reverse, 5′-gcagtagctgcgctgataga-3

From

https://www.google.com.hk/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=0ahUKEwiRyPrR677RAhVFpZQKHU6PAKoQFggmMAE&url=http%3A%2F%2Fwww.nature.com%2Fonc%2Fjournal%2Fv26%2Fn51%2Fextref%2F1210535x5.doc&usg=AFQjCNEeiLkV8iN3hVdAD6yv0I-nrZAkVw

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