I want to isolate cells from bile to extract DNA and evaluate some biomarkers. I did not any clear method in the literature to do it. Did anybody can give me some idea?
Perhaps this will help, Dilute the bile 1:1 with sterile distilled water, centrifuge at 18000 g for 15 min. and discard the supernatant. Mix and resolve the pellet in sterile water and use this for input of a DNA extraction Kit
I had a look in article that has been suggested. In that work they extract bacterial DNA by that method. Do you think it should be suitable for human cells (and DNA)?