I have developed bioanalytical method for estimation of Lamotrigine in human plasma using LC-MS/MS and now I am going to apply my method to Therapeutic drug monitoring and Pharmacokinetics in epileptic patients.
I had already taken 3 patients blood samples at different time interval and done the same analysis by LC-MS/MS.
Now, the problem is whatever Lamotrigine concentarions getting from the patients sample analysis was too high, it was out from the my linearity range upto 30- 40 times. I had taken blood samples at 0, 0.5, 1,2,3,4, 12 and 24 hrs from patients.
Patient dose profile:
drug given
Lamictal 100mg ....... 1---0---1
so, what can i do....for better analysis?
Hi Kashyap.
I agree with Tom Tomian. You have a good problem in your work, the method require a less sensitive than you need. You could try inject a low volume or you can try change the mass spectrometer setup lower your analytical signal.
Regards
At first, Thanks all of you for valuable suggestion.
I assure you that developed method were within the cmax range. In addition to that, patients were taking this drug (lamictal 100mg, twice a day) regularly.
now, I was doing clinical application of my developed method and i have to submit the data to patients and doctors also.
so, if i am dilute patient samples then how could i get the exact concentration of drug in patients. if i would apply dilution factor then again same problem arise.
Kashyap.
You could dilute your samples with the same matrix your method was validated. You could validate the dilution process, may be in several concentration level, if you thinks necessary.
For calculate the final concentration of samples, you use the dilution factor.
Another way is validate your method in another linear range. But will require more time.
I hope help you.
Liquid-liquid extraction (200 ul) with acetonitrile (400 ul) followed by vacuum evaporation to dryness and then reconstitute with mobile phase (300 ul)
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