Any suggestion for doing antioxidant activity using nanoparticle sample?

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Gene editing using Cas9 RNP?

Hi everyone, Im trying targeted gene editing in citrus protoplast through PEG-mediated transformation. Im using sgRNA+Cas9 RNP complex for DNA free genome editing. But im not successful in...

06 July 2019 1,471 1 View

How to design and insert my target sequence into sgRNA scaffold?

Dear All, I want to design sgRNA target sequence and insert into scaffold portion already present in pRGEB31 binary vector. Any experimental protocol for that? I want to knock out a disease...

05 June 2018 4,918 2 View

Anyone knows in vitro digestion of DNA with Cas9?

I am going to check my gRNA efficiency by in vitro digestion of DNA with Cas9. I read the protocol from NEB website but have some doubts regarding that. I want to know whether i should do in vitro...

05 June 2018 9,932 0 View

Standard protocol for plant tissue preparation to visualize GFP?

Dear Researchers, I am doing transformation in cotton plant. Now I want to check GFP expression in transformed tissue. Can anyone suggest a standard protocol for preparing tissue sample to...

10 November 2016 1,417 5 View

Is vacuum infiltration really effective for agrobacterium mediated transformation in seeds of recalcitrant plants?

Dear Researchers, Im trying vacuum infiltration for seed transformation of a recalcitrant plant. I tried different time durations of vacuum infiltration (1 min to 10 min), culture density...

08 September 2016 6,839 3 View

Antioxidant enzymes in cotton seedlings?

Dear Researchers, Is it possible for cotton seedlings (10 days old) to produce antioxidant enzymes in cotyledons and other parts under short duration (6h,12h and 24h) of salt and drought...

06 July 2016 4,977 3 View

Any suggestion regarding protein sample preparation for 2D gel electrophoresis?

Hi Researchers, Currently I am doing 2DGE to identify differential protein expression (unknown) in plant tissues under various abiotic stress conditions. I am following phenol extraction...

05 June 2016 407 4 View

Protein expression in cotton seedlings under abiotic stress?

Dear all, I have planned to study differential expression of proteins through 2-D electrophoresis (not any target protein) in 7 days old cotton seedlings under different abiotic stress (NaCl,...

03 April 2016 8,239 7 View

Does bubble formation affect separation of proteins in 2D electrophoresis?

Dear Researchers, I was preparing to run 2nd dimension (SDS PAGE) after finishing 1st dimension (isoelectric focusing). After finishing everything perfect, i was sealing top of the IPG strip...

03 April 2016 557 13 View

Good protocol for silver staining?

Hi researchers, for the first time i am going to do silver staining of SDS PAGE. can anyone suggest me a good protocol for doing that? Thanks in advance.

02 March 2016 1,980 6 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View