Any substitute for raffinose during galactose induction?

More Aswathy Narayanan's questions See All

H2/CO ratio calculation in dry reforming?

How to calculate H2/CO ratio in dry reforming? This question is for my friend. The ratio should come around 1.0. But the ratio coming 35, 40, 48. CO peak is higher and H2 peak is smaller. What is...

31 January 2021 7,788 3 View

We know transfer learning is used immensely now a days. Shall we use a model trained on coco dataset for cancer detection.?

The answer is Yes. The cancer images eg: histopathology images are entirely different from coco dataset images like cat,, dog, van etc. As the coco dataset features are also different from cancer...

15 December 2020 2,625 5 View

For dissolving haloalkanes which is best solvent - polar or nonpolar?

For dissolving haloalkanes which is best solvent - polar or nonpolar? How much quantity of solvent is required if the solublity is unknown.

12 October 2020 3,447 4 View

Why the color of dichloropropane changed?

The color of dichloropropane changed from colorless to brown color during distillation. The actual color of dichloropropane is colorless. Why the color changed. Thank you

27 September 2020 3,216 3 View

What is the importance of segmentation of histopathology images prior to classification?

I wanted to know how a classifier model will be affected by a poorly segmented image?

21 September 2020 8,205 14 View

Conversion of Dichloroalkane into Alkanediols?

I need to convert 1,2-dichloralkane to alaknediol using reflux condenser. The dichloroalkane is insoluble in water. Hence i need to take methanol/ethanol as solvent. For substitution reaction i...

14 September 2020 6,292 3 View

How can I get access to the travaux preparatories of international conventions related to Intellectual Property Rights?

For instance, the 'Historical Archives' section of the 'Audio Visual Library of the International Law' website gives access to the negotiation documents related to various instruments of...

07 June 2020 3,135 3 View

I have two H and E stained histopathology image datasets of breast. I wanted to use both for training a single algorithm. So how can i do this?

Should I combine both the datasets and train the ML algorithm or should I need to train the algorithm separately with the datasets and test on a single set.The test set is the combination of both...

08 May 2020 6,648 5 View

Can anyone refer me the spectral library associated for identification and classification of mangrove species in remote sensing data?

Species level classification of mangrove around east coast

02 January 2020 7,810 3 View

Bulk amorphous, but nano crystalline?

My otherwise amorphous material is showing strong XRD peaks when in its nanoparticulate form...cant really guess the reason...need help

02 January 2018 3,668 6 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Barry G. Hall

I have a vague recollection that methyl-galactoside is an inducer in E. coli. MeGal is cheap, so it might be worth checking out

Dmitri Churikov

Glucose represses GAL1 promoter, so if you culture your cells in raffinose (instead of glucose) then you should get better induction of your gene with galactose.

Aswathy Narayanan

Thank you all...