Any reference for Alcian blue staining of the hyaluronic acid in the mice skin sections?

22 April 2021 1 5K Report

I would like to quantify hyaluronic acid in the skin section of WT vs Mutant mice. Is Alcian blue staining is a good pick? Any recommendations are appreciated.

Thanks

Alexandr Chernov

Dear Vinod!

Please you look at the article:

Article Extrinsic ageing in the human skin is associated with altera...

(attached)

Skin tissue specimens

Figure 1. Differential expression of GAG in photoexposed and

photoprotected skin. (a) Representative figures of the electrophoretic

mobility of total GAG (4 lg of uronic acids) isolated and purified from

photoexposed (Ex) and photoprotected (Pr) skin tissue specimens from

two individuals on cellulose acetate membranes stained with Alcian

blue. (b)

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