Any recommendations on IHC-paraffin/IF antibodies to detect NK cells and HLA-I?

Juan A. Marín-Jiménez @Juan-Marin-Jimenez

04 November 2021 1 1K Report

I am working with patient-derived tumor samples and I would like to detect CD56/NCAM1+ cells as a marker for NK cells and MHC-I molecules (HLA-ABC) in the same slide.

Could you recommend chromogenic vs fluorescent detection and, if possible, specific antibodies to build my panel?

Many thanks

Jean-Martin Lapointe

CD56 will stain NK cells, but other immune cells as well, so it's not a specific marker. NKp46 is a more specific marker for NK cells. Not sure you'll be able to find an IHC antibody for all MHC-I versions, and if you did it would probably stain all of the cells in the sample, so not sure what you are trying to do with that combo.

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