Any recommendations for anti E. coli (UTI89) and/or anti P. mirabilis antibodies?

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How to make a paste in a soft tablet form which is easily chewable n spread in the mouth completly?

the tablet should be compressed n the mixture of paste shouldnt be heated much

16 October 2023 9,718 1 View

Use of 'nsolve' of sympy to find the solution of system of non-linear algebraic equations?

Hello eveyone, I am trying to use nsolve of sympy to find solutions of system of non-linear algebraic equations. My system has three non-linear equations with three unknowns. Each of these...

01 March 2023 4,481 0 View

Can I find molecular weight of PLGA based formulation with respect to time when subjected to in-vitro release using viscosity as a measurement tool?

I want to find out degradation rate of PLGA formulation w.r.t. time for which change in initial molecular weight would be beneficial. Moreover, I don't have GPC facility. Please help me if anyone...

11 November 2021 3,717 1 View

How can I run movestay command?

I used the following command: movestay (lnTotalincome = HHsize h1sex TLU yrcop tland tprod) (ccert = HHsize h1sex TLU yrcop tland tprod), select (train = h1edu) But this doesnot work and I get...

01 November 2020 1,367 2 View

Why CSI Bridge/SAP2000 showing Plastic Hinge in Bridge Pier even if Pier Moment Value has not reached to it's Mp Value?

Hello every one, I am doing analysis of Bridge in which elastomeric bearing is modeled as link element between Deck and pier bent. Pier bent portal consist 2 pier and one cap beam on which...

12 August 2020 8,826 2 View

What are the best tools and study protocol to study the beneficial soil microorganism and the symbiotic agents?

I am going to conduct the field survey to collect the major soil living organisms, beneficial insects, natural enemies, and decomposers and symbiotic agents. For each study, I am looking for...

28 July 2020 8,509 3 View

How to write a code for single precision floating point multiper using verilog with the help of xilinx?

I wanted to design single precision floating point multiper using verilog plz provide me a source code for the same

11 April 2020 7,165 2 View

We are studying the post listing behaviour of the securities listed to NSE. How to link Promoter's holding, fim's age, issue size with underpricing?

Lot of parametres impact the post listing behaviour of securities. How these parametres, collectively affect the behaviour? After IPO being floored, how can we assess these parametres with...

19 February 2020 1,764 3 View

How to perform simulations of electrostatic pull-in instability of beams in ANSYS?

Hello everyone, I want to perform electrostatic pull-in instability simulations of beams with various fixity conditions in ANSYS to get pull-in parameters. I want to compare these results from...

10 January 2020 5,075 0 View

Bolted Joints Design Guidelines for Plastic & FRP composites without inserts?

Hello, I am interested in bolted joint for plastic & FRP composites. Can you recommend me any research or book related to this? Do you strictly include metal inserts & compression...

02 January 2020 2,613 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View