I am looking for a IoT simulator ?

I am working on a university project so, I m looking for a good Simulator compatible with LoRa and LoRaWAN protocol to simulate an IoT scenario. Thanks in Advance

20 February 2021 6,352 6 View

How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View

What is the Role of temperature In Hummers method for the preparation of graphene oxide?

04 January 2021 5,033 2 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

What is the appropriate media for WI-38 cell line?

Iam going to culture WI-38 ( a normal lung cell line), it is the first time for me to deal with this cell line so i want to know the most suitable media for this cell line? In addition to, if i...

18 December 2020 6,542 3 View

Can i use an expired fetal bovine serum?

I have fetal bovine serum expired from many years ago but it kept at -200C during all this period and did not expose to cycles of freezing and thawing. So, can i use it for cell culturing? In...

18 November 2020 1,237 7 View

How to implement LEACH-C (LEACH centralized) algorithm for CH selection in WSNs?

I am currently working on a project that is based on optimizing cluster head (CH) selection for energy-efficient wireless sensor networks (WSNs) using evolutionary search techniques. For the...

11 October 2020 5,814 3 View

How can i solve the problem appeared during autogrid run using cygwin?

This is the first time for me to use the autodock software. I used autodock tools 1.5.6 I prepared pdbqt file for my ligand and my target and also file for grid parameters and docking parameters,...

02 September 2020 5,914 2 View

How do you think our Architectural spaces would change after COVID 19?

How do you think our Architectural spaces would change after COVID 19?

10 May 2020 3,172 27 View

What is the suitable statistical analysis to test the relationship between two diseases from 5 different regions?

I have only the total number of cases for each diseases (measured by millions) and I'm trying to find a significance relationship for their coexistence throughout the 5 regions...

24 March 2020 4,397 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

What makes yellow color appear in phosphorus sample after autoclave digestion?

I was using autoclave for digesting water samples for Total phosphorus measurement with ascorbic acid method. The autoclave was set to perform 2 hours digestion and around 121 Celsius degrees. I...

25 January 2021 206 3 View

SDS-PAGE aggregation problem?

I ran SDS-PAGE with 14% lower gel, 4 % upper gel, having brushing, pre-running empty gel for 30 min. The protein samples had been centrifuged with 14,000g for 10 min, and then the supernatants...

20 January 2021 172 3 View