Hi,

We are trying to extract DNA from a potting mix (peat moss + perlite + soil) but had some issues with some of our samples. We are using the Qiagen PowerSoil extraction kit, and have modified a bit the protocol to include an incubation step and decrease the final elution volume. It has worked well in general but some samples are quite recalcitrant.

We have also modified the initial sample weight to be placed in the tube, increasing and decreasing it, but it does not seem to have an effect.

Does anyone have a suggestion as to how to improve our DNA yield? Thanks a lot!