Dear All,
I am wondering if you know of any gating strategies for analyzing T and B cell populations in Murine blood samples collected in heparin-coated tubes.
As far as I know,
100ul of blood, stained with appropriate antibodies should be lysed, fixed, and subjected for analysis.
For your information, I have Four color FACS machine and would like to know the antibody panels used for analysis along with the gating strategies would be a great help.
For instance, I was thinking of the following panel, CD3, CD4, CD8, B220- for the T cell population
and CD3-, B220+, CD19+ for B cell population.
Please enlighten me if I am wrong.
Thanks in advance.
Hello!
https://onlinelibrary.wiley.com/doi/full/10.1002/eji.201646632?hootPostID=c8f3c653434c521d665f2f7d0e92447f
Here it is.
https://www.nature.com/articles/s41598-019-42913-4
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies