Is there a common protocol to deparaffinization of formalin-fixed paraffin embedded (FFPE) tissue prior to proteomics conduction?
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
If i did a same experiment for a 2 populations and i need to publish this work in a 2 different papers can i use the same word in the writing of these 2 papers or this would considered as...
04 February 2021 4,648 4 View
Many steps can be taken to make the year 2021 good for humanity. For instance, if powerful and rich countries decide and spend some part of their resources for the improvement and betterment of...
30 December 2020 830 4 View
Many bad and good things happened for global community in 2020. However, the present question is about one top most worst thing and one top most good thing that happened in the world in 2020 and...
30 December 2020 6,262 3 View
I want to exract genomic DNA from skin and gills of fish.
25 December 2020 6,118 3 View
Several environmental experts have warned about the global warming and its effects on different ecosystems. It may also cause large scale melting of continental and alpine glaciers. If this...
23 December 2020 4,630 3 View
Dear all, I recently bought a RTX3080 and was unable to start the NPT and production steps due to lincs warning (my old gtx 1080ti card ran normally, using the same .mpd), I tried several...
18 December 2020 3,333 2 View
Dear All, Please I has accidentaly removed 22 publications from RG my profile in the mail that you send me today, please put again those publicatios in my profile. Best regards,
13 December 2020 9,981 4 View
Agriculture provides for almost all the basic needs essential for survival and industries provide mostly the luxury commodities required for comfortable life. However, some countries are heavily...
07 December 2020 2,153 15 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View
How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex
22 February 2021 5,667 2 View
Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...
18 February 2021 4,565 3 View
I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...
14 February 2021 6,688 3 View
I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...
10 February 2021 3,113 3 View
RBCs have auto-fluorescence, I want to detect that auto-fluorescence in paraffin embedded tissue samples. Any suggestions?
08 February 2021 9,357 1 View
Hello fellow academics, For my Final Year Project, I am looking into different ways of modelling the interface between PVA and gelatin. I have successfully modeled the interface using the "tied"...
04 February 2021 8,698 1 View
31 January 2021 8,678 3 View
Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo
26 January 2021 4,165 3 View