Is there a common protocol to deparaffinization of formalin-fixed paraffin embedded (FFPE) tissue prior to proteomics conduction?
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...
02 July 2024 9,807 3 View
What should the requirement be for an all-inorganic perovskite solar cell? What should be the nature of its layer? If we use any organic layer in an all-inorganic perovskite active material-based...
24 May 2024 3,355 2 View
Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...
21 May 2024 2,171 0 View
I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...
12 May 2024 1,045 4 View
I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...
29 April 2024 8,231 1 View
As known, the spin exchange coupled Mn4+ leads to a narrowd singal, at ca. g=2.006 with the line width about 500 gauss. Why the birnessite MnO2 dispalys such wide spectrum? Is any other exchange...
23 April 2024 6,351 3 View
What requirements (or qualities) are most important to be a good university professor?
22 April 2024 3,009 5 View
I'm a current medicine student and I'm in my first scientific initiation in my graduation. Currently, me and my professor are having some troubles with contamination in our cells. This is a bottle...
18 April 2024 6,899 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
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24 July 2024 1,559 1 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Many have criticized Noam Chomsky’s theory of language (e.g., Pinker as described in Sihombing 2022), but the most effective criticisms have come from Daniel Everett, given that Chomsky (according...
15 July 2024 492 4 View
Immunofluorescence staining of mouse spleen paraffin sections showed strong fluorescence signals in red blood cells in the control section without antibodies. Is it autofluorescence of red blood...
02 July 2024 827 3 View
Must I cut them immediately with a microtome or can I keep them and use them over time?
30 June 2024 4,498 2 View
Can anyone recommend polyclonal or monoclonal antibodies for Tet methylcytosine dioxygenase 2 (TET2) Tet methylcytosine dioxygenase 3 (TET3) DNA methyltransferase 3 beta (DNMT3B) Ideally, they...
27 June 2024 3,557 1 View
Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...
25 June 2024 4,602 0 View
Sample type: Corn leaves and stem Bean leaves and stem
22 June 2024 2,082 3 View
I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.
05 June 2024 3,831 0 View