It is different from ribavirin or G in terms of base pairing. It will be interesting to evaluate its mono-, di- and triphosphate, to test the importance of ribofuranosyl moiety. Very nice findings.
the conformational similarity with G and A has not been tested to date but with a strategy of oligo synthesis, it will be necessary
I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...
08 June 2022 2,467 0 View
The purpose is to generate in vitro spheroids from the biopsy samples. Storage at 4 degrees Celsius is due to transportation. Thank you.
30 April 2021 2,917 2 View
Cre recombination efficiency often impacts activation of Cre/LoxP dependent transgenes. We have recently encountered problems with recombination in certain cell types in vivo, especially with...
11 August 2019 7,555 3 View
Hi All, Can MeDIP-seq (methylated DNA immunoprecipitation-sequencing) be performed on DNA previously stained with DAPI or Hoechst? If not, are there any DNA stains compatible with the...
13 November 2016 2,637 1 View
What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...
14 June 2016 8,558 10 View
Hi All, I'm looking for an antibody directed against mouse nuclei (not cell type-specific), preferably directed to component(s) of nuclear envelope, and being a monoclonal. Antibody is intended...
06 June 2016 4,184 1 View
Hi All, I need to FACS-sort formaldehyde-fixed nuclei from mouse brain tissue. What is the best strategy for staining of intranuclear protein for immunolabeling for FACS (staining of soluble...
14 May 2016 5,244 3 View
Hi All, I was just wondering if anyone tried to use any of the Miltenyi beads in tubes instead of columns, with a standard magnets compatible with the "tube" magnetic separation format. My guess...
03 May 2016 304 3 View
Should using Taq polymerase be avoided when when doing SOE PCR? I would assume that, due to addition of non-template A and lack of exonuclease activity, it would generate mutant extension...
18 July 2015 4,137 5 View
I'm looking for an IL-8 mouse specific ELISA but can't find any (at least via mighty google). I'd appreciate any suggestions. Lech
08 January 2015 943 4 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello, I am Master student under molecular biology. I want to find the Shine-Dalgarno sequence after performing a BLAST search in TargetRNA2. Could you please suggest a web tool to predict the...
07 July 2024 9,598 1 View
Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...
07 July 2024 8,626 2 View
I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...
02 July 2024 612 6 View
I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...
26 June 2024 4,522 0 View
I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...
24 June 2024 6,079 4 View
I'm measuring the setting time of a calcium phosphate cement using an apparatus that records how much resistance a needle (connected to a load cell) encounters over time. This process provides...
24 June 2024 7,534 5 View
Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...
16 June 2024 5,876 5 View
I'm following a paper for their HPLC methodology, but I'm having a hard time understanding how to make mobile phase A. To quote: "Mobile phase A is a mixture containing 0.2% potassium dihydrogen...
13 June 2024 8,321 1 View
The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?
10 June 2024 3,970 2 View