Any PCR additive that can be used for direct whole blood PCR?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Tamas Kerekes

Hi,

I've suggest you the following short article:

http://www.epibio.com/docs/default-source/forum-archive/forum-07-4---direct-pcr-from-whole-blood.pdf?sfvrsn=6

Or this kit can be useful, but more expensive than in-house methods:

https://www.thermofisher.com/order/catalog/product/F547S

Bests,

Tamas

Or maybe this paper can be useful:

Article Direct polymerase chain reaction from whole blood without DN...