I've tried NP-40, formamide, tween, trition, trehalose and gelatin. these thing didn't work in the whole blood direct PCR. So, is there any suggest about PCR additives which can improve the results of direct PCR on whole blood without extraction?
Hi,
I've suggest you the following short article:
http://www.epibio.com/docs/default-source/forum-archive/forum-07-4---direct-pcr-from-whole-blood.pdf?sfvrsn=6
Or this kit can be useful, but more expensive than in-house methods:
https://www.thermofisher.com/order/catalog/product/F547S
Bests,
Tamas
Or maybe this paper can be useful:
Article Direct polymerase chain reaction from whole blood without DN...
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