Dear Ayad Palani,

Look the link. May be useful.

http://ijcsi.org/papers/IJCSI-8-6-1-68-72.pdf

http://connection.ebscohost.com/c/articles/100087693/blind-recognition-recursive-system-convolutional-codes

https://books.google.az/books?id=yrGEM3YSvIAC&pg=PR8&lpg=PR8&dq=blind+recognition+algorithm+of+Turbo+codes%22+in+MATLAB?&source=bl&ots=xJtbxUZ8zg&sig=daVDNiHz_SdEMFiiJNuh0PKknGM&hl=ru&sa=X&ved=0ahUKEwj875Sele3KAhXJYpoKHUjICbAQ6AEIRjAG#v=onepage&q=blind%20recognition%20algorithm%20of%20Turbo%20codes%22%20in%20MATLAB%3F&f=false

Regards, Shafagat

is this the sample buffer? I think I normal get a very mild bluish green tinged buffer

hello Mr. Palani, i know what do you describe and it is a problem! First, ensure that the acetic buffer is on room temperature. Then put 1 ml of TPTZ solution in 10 ml buffer and then add 1 ml of FeCl solution. This working solution takes a straw-brown color in 37o C.

i had this blue-purple color when my acetic buffer was cold or had been made time ago.

Good luck!

the test of FRAO antioxidant method assay is clair and sample, you can founded  many references and publication. You founde our method utilisation: 

Measurement of ferric reducing power (FRAP assay): Briefly,  the FRAP reagent contained 2.5 mL of 10 mM tripyridyltriazine (TPTZ) in 40 mM HCl, 2.5 mL of 20 mM FeCl3 and 25 mL of 0.3M acetate buffer (pH 3.6), was freshly prepared. A volume 0.2 mL, of ethanolic extract) various concentrations) or standard was mixed with 1.8 mL of freshly prepared FRAP reagent. The absorbance of each sample solution was measured at 595 nm19. For the calibration curve, FeSO4 was prepared in same solvent extraction in the range of 100–700 µM and querecetin was used as positive controls. The results were expressed as mg/ml of Fe(II), using the equation obtained from the calibration curve of FeSO4: Y = 6.908x , R2 = 0.998.

Please see the following link

http://www.sciencedirect.com/science/article/pii/S0926669013004603

thank you all. my question is , when I mix TPTZ with FeCl3 a blue color are formed before the addition of sample, is there a problem with that??

hi.i check my method.

Use this method now.add the buffer at first. second TPTZ   and the end FECL3.

was it helpful?let me know.

Yes this indicstes that the FRAP may be reacting before your test

Attached is an article that may be helpful. There is the description of FRAP assay on that:

Article High-throughput assay comparison and standardization for met...

Best regards

B.Ferreira