I have a Serine doublet in my protein sequence. I am not able to find a sequential connection between these two. Is there any NMR experiment which gives all serines present/absent in a single spectrum ?
1) Is this SerSer in a loop or at the end of structured region (from theoretical secondary structure prediction). It could be that you are not seeing one of the Ser due to relaxation properties. Try collecting another spectra at a different temperature ( you might get lucky).
2) Is it possible that both Ca and Cb of both Ser are overlapping?
if you have a 13C/15N labeled protein then the HNCACB or HNCOCACB experiments are best to detect serines. This is due to the unique C-beta chemical shift of Ser andThr. In absence of labeling a HSQC or NOESY HSQC might help.
Dear Sujeesh,
as a add on to Clemens's answer...you could try a multiplicity edited HSQC. CH2 moieties in the Cb chemical shift range of Ser/Thr should be identifyable...This also should work at natural abundance if you do not have a 13C or 15N label.
Regards
Alfred
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