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Questions related from Sujeesh Sukumaran
I have 20% (w/w) formaldehyde-d2 in water. I want to calculate the isotopic purity using NMR. But I am getting 3 to 4 peaks corresponding to different polymers of methylene glycol. How to find the...
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What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?
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If I design a peptide for preventing aggregation of Abeta 42 peptide, what are aspects should I consider if I want to suggest it as drug for Alzheimer disease. What all are the properties should...
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I have a calcium binding protein. To make it calcium free i did dialysis with EDTA containing buffer. But how to make sure whether a protein is calcium free ?
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I have a peptide which can form fibrils upon heating. I wish to find some application to that. What are the experimental conditions should I try to find whether my peptide form a hydro gel ? How...
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Why do we generally prefer cells like DH5alpha or top10 for increasing plasmid amount and isolation? Why are we using BL21 for protein expression? Why not DH5alpha?
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I have a protein of 277 aminoacids. I want express a truncated version of that without first 22 aminoacids from N-terminal and also the sequence should start with a cysteine residue. Is there any...
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