Having some issues with our library of bacteriophage binding to our polyvinyl chloride plates. Any methods to help reduce binding to the plate?
Mr. William Douglas Rees,
how you isolated your bactriophage/ what method you used/ what type of phage ? and give more details for how you found your phage binding to polyvinyl chloride plates ?
Nattan Stalin,
Sorry for the late response.
I am attaching a file of our ELISA protocol
We are using M13 bacteriophage
We found that our phage was binding to the PVC plates because we had a high number of non-specific binding. Our binding to HSA was low.
Thanks,
Bill
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