Any methods for reducing plastic binding of bacteriophage to polyvinyl chloride plates?

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Nattan Stalin

Mr. William Douglas Rees,

how you isolated your bactriophage/ what method you used/ what type of phage ? and give more details for how you found your phage binding to polyvinyl chloride plates ?

William D Rees

Nattan Stalin,

Sorry for the late response.

I am attaching a file of our ELISA protocol

We are using M13 bacteriophage

We found that our phage was binding to the PVC plates because we had a high number of non-specific binding. Our binding to HSA was low.

Thanks,

Bill