William D Rees

4 Questions 14 Answers 0 Followers

Questions related from William D Rees

Do I need to add IL-2 for naive T-cell growth during a DC T-cell co-culture?

Hey Everyone, I'm trying to plan out a DC T-cell co culture and I'm not sure if i'll need to add IL-2 to my culture to support my T cells during my incubation (4-5 days for proliferation assay)....

25 January 2019 4,715 6 View

How much calcium should i add to clot whole blood that has been collected in a tube containing EDTA?

Hi everyone, I would like to collect some serum from my whole blood sample to test performing an ELISA. How much calcium should be added to clot my blood that has been collected in a tube...

01 April 2016 4,788 7 View

Does anyone know a good blocking buffer for ELISA?

For my ELISA, i am using PBS + 5% non fat dry milk as a blocking buffer. I have also tried using PBS + 1% Casein in TBS as a blocking buffer. I am still getting non specific binding to my...

13 January 2016 8,625 14 View

Any methods for reducing plastic binding of bacteriophage to polyvinyl chloride plates?

Having some issues with our library of bacteriophage binding to our polyvinyl chloride plates. Any methods to help reduce binding to the plate?

18 January 2015 8,152 2 View