Robust cAMP assay kit for Gi coupled GPCRs ?

Dear all, Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs? I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins)...

09 October 2017 265 3 View

How to get rid of colony in negative control after blunt end digestion followed by ligation?

I have digested my vector and PCR product with restriction enzymes A and B (both blunt end cutters). For ligation I have either the vector or vector + insert. Now as it is a blunt end digestion,...

02 March 2016 8,850 4 View

Direct protein estimation from cultures cells in a 96 well plate?

01 February 2016 7,864 7 View

what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification?

Dear All, I want to know what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification? I know the capacity of the two are different, but is that the...

31 December 2015 8,796 4 View

How do I normalize my MTT assay for reduced cell number in treated sample if my drug inhibits cell division?

Hello All, I am doing a MTT assay where because of drug treatment , the no. of cells in treatment is reducing to half of control. Now, after MTT assay how do i know the reduction in absorbance is...

09 October 2015 1,758 12 View

Why does 0.32M sucrose prevent clathrin-mediated endocytosis?

why does 0.32M sucrose prevent clathrin-mediated endocytosis?

05 June 2015 9,519 0 View

Bioinfo tool for determination of metabolic pathways?

Is there any bioinformatics tool to determine any metabolic pathway given the components?

01 February 2014 5,927 4 View

How to prevent inner glass plate breaking during SDS PAGE in PROTEAN II xi cell?

I am using BIO-RAD PROTEAN II xi cell for protein gel electrophoresis. I am using 15mA constant current for first two hours then 35mA constant current for the rest of the run. My inner glass plate...

03 April 2013 5,393 28 View

How does 67% Acetic acid in 33mM MgCl2 dissolves ribosomal proteins but precipitates rRNA?

Ribosomal proteins from ribosome is extracted best by 67% acetic acid in presence of 33mM MgCl2. What is the mechanism and how does acetic acid helps here? For reference : 1....

03 April 2013 6,184 1 View

Ribosome digestion with RNase A giving RNA fragment with very low mobility in gel.

I am digesting ribosome with RNase A to remove rRNA and release the ribosomal proteins. When I run a 1% agarose gel after reaction, compared to my control (without digestion), in the experiment...

03 April 2013 4,461 10 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View