Any literature on cholesterol content of plasma membrane in various human cells?

Dear all, Could you suggest any review/paper for a comprehensive list of cholesterol content of plasma membrane in various human cells ? For example, difference in membrane cholesterol content of...

08 September 2018 1,009 2 View

Robust cAMP assay kit for Gi coupled GPCRs ?

Dear all, Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs? I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins)...

09 October 2017 265 3 View

How to get rid of colony in negative control after blunt end digestion followed by ligation?

I have digested my vector and PCR product with restriction enzymes A and B (both blunt end cutters). For ligation I have either the vector or vector + insert. Now as it is a blunt end digestion,...

02 March 2016 8,850 4 View

Direct protein estimation from cultures cells in a 96 well plate?

01 February 2016 7,864 7 View

what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification?

Dear All, I want to know what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification? I know the capacity of the two are different, but is that the...

31 December 2015 8,796 4 View

How do I normalize my MTT assay for reduced cell number in treated sample if my drug inhibits cell division?

Hello All, I am doing a MTT assay where because of drug treatment , the no. of cells in treatment is reducing to half of control. Now, after MTT assay how do i know the reduction in absorbance is...

09 October 2015 1,758 12 View

Why does 0.32M sucrose prevent clathrin-mediated endocytosis?

why does 0.32M sucrose prevent clathrin-mediated endocytosis?

05 June 2015 9,519 0 View

Bioinfo tool for determination of metabolic pathways?

Is there any bioinformatics tool to determine any metabolic pathway given the components?

01 February 2014 5,927 4 View

How to prevent inner glass plate breaking during SDS PAGE in PROTEAN II xi cell?

I am using BIO-RAD PROTEAN II xi cell for protein gel electrophoresis. I am using 15mA constant current for first two hours then 35mA constant current for the rest of the run. My inner glass plate...

03 April 2013 5,393 28 View

Ribosome digestion with RNase A giving RNA fragment with very low mobility in gel.

I am digesting ribosome with RNase A to remove rRNA and release the ribosomal proteins. When I run a 1% agarose gel after reaction, compared to my control (without digestion), in the experiment...

03 April 2013 4,461 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View