17 Questions 22 Answers 0 Followers
Questions related from Parijat Sarkar
Dear all, Could you suggest any review/paper for a comprehensive list of cholesterol content of plasma membrane in various human cells ? For example, difference in membrane cholesterol content of...
09 September 2018 1,093 2 View
Dear all, Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs? I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins)...
10 October 2017 316 3 View
I have digested my vector and PCR product with restriction enzymes A and B (both blunt end cutters). For ligation I have either the vector or vector + insert. Now as it is a blunt end digestion,...
03 March 2016 8,892 4 View
Dear All, I need a protocol for estimation of protein from cell cultured in a 96 well plate. I want to lyse the cells in the same well and then do a protein estimation (preferably in the same...
02 February 2016 7,916 7 View
Dear All, I want to know what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification? I know the capacity of the two are different, but is that the...
01 January 2016 8,845 4 View
Hello All, I am doing a MTT assay where because of drug treatment , the no. of cells in treatment is reducing to half of control. Now, after MTT assay how do i know the reduction in absorbance is...
10 October 2015 1,816 12 View
why does 0.32M sucrose prevent clathrin-mediated endocytosis?
06 June 2015 9,598 0 View
Is there any bioinformatics tool to determine any metabolic pathway given the components?
02 February 2014 5,987 4 View
I am using BIO-RAD PROTEAN II xi cell for protein gel electrophoresis. I am using 15mA constant current for first two hours then 35mA constant current for the rest of the run. My inner glass plate...
04 April 2013 5,546 28 View
Ribosomal proteins from ribosome is extracted best by 67% acetic acid in presence of 33mM MgCl2. What is the mechanism and how does acetic acid helps here? For reference : 1....
04 April 2013 6,236 1 View
I am digesting ribosome with RNase A to remove rRNA and release the ribosomal proteins. When I run a 1% agarose gel after reaction, compared to my control (without digestion), in the experiment...
04 April 2013 4,513 10 View
I am trying to digest RNA with RNase A. I want to digest the RNA for 10 minutes. How do I immediately stop the reaction?
09 September 2012 1,049 2 View
Is there any tool (like available for DNA restriction mapping at NEB) for restriction of RNA? It will be fantastic if I can provide the secondary structure of the RNA and the enzyme cuts...
08 August 2012 4,315 11 View
Are there any alternatives to iodoacetamide in 2D, used in equilibration buffer 2? I am using BIORAD Readystrip IPG Strip.
08 August 2012 5,894 18 View
I am facing problem in setting up in vitro translation reaction. It looks like I need an energy generating system like phosphoenol pyruvate and pyruvate kinase . There are kits available (NEB)...
07 July 2012 6,478 4 View
07 July 2012 7,569 2 View
Can anybody suggest a software where I can specify one gene sequence, forward and reverse primer sequence and then the program will run a PCR with parameters set by me? All the available...
06 June 2012 9,529 1 View