Any literature on cholesterol content of plasma membrane in various human cells?

Dear all, Could you suggest any review/paper for a comprehensive list of cholesterol content of plasma membrane in various human cells ? For example, difference in membrane cholesterol content of...

08 September 2018 1,009 2 View

Robust cAMP assay kit for Gi coupled GPCRs ?

Dear all, Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs? I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins)...

09 October 2017 265 3 View

How to get rid of colony in negative control after blunt end digestion followed by ligation?

I have digested my vector and PCR product with restriction enzymes A and B (both blunt end cutters). For ligation I have either the vector or vector + insert. Now as it is a blunt end digestion,...

02 March 2016 8,850 4 View

Direct protein estimation from cultures cells in a 96 well plate?

01 February 2016 7,864 7 View

what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification?

Dear All, I want to know what is the basic advantage of using Ni NTA agarose over Ni NTA superflow for membrane protein purification? I know the capacity of the two are different, but is that the...

31 December 2015 8,796 4 View

How do I normalize my MTT assay for reduced cell number in treated sample if my drug inhibits cell division?

Hello All, I am doing a MTT assay where because of drug treatment , the no. of cells in treatment is reducing to half of control. Now, after MTT assay how do i know the reduction in absorbance is...

09 October 2015 1,758 12 View

Why does 0.32M sucrose prevent clathrin-mediated endocytosis?

why does 0.32M sucrose prevent clathrin-mediated endocytosis?

05 June 2015 9,519 0 View

How to prevent inner glass plate breaking during SDS PAGE in PROTEAN II xi cell?

I am using BIO-RAD PROTEAN II xi cell for protein gel electrophoresis. I am using 15mA constant current for first two hours then 35mA constant current for the rest of the run. My inner glass plate...

03 April 2013 5,393 28 View

How does 67% Acetic acid in 33mM MgCl2 dissolves ribosomal proteins but precipitates rRNA?

Ribosomal proteins from ribosome is extracted best by 67% acetic acid in presence of 33mM MgCl2. What is the mechanism and how does acetic acid helps here? For reference : 1....

03 April 2013 6,184 1 View

Ribosome digestion with RNase A giving RNA fragment with very low mobility in gel.

I am digesting ribosome with RNase A to remove rRNA and release the ribosomal proteins. When I run a 1% agarose gel after reaction, compared to my control (without digestion), in the experiment...

03 April 2013 4,461 10 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Please suggest me the simplest way to understand the in-silico analysis of any gene?

I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?

22 February 2021 8,879 2 View

Is there a Constitutive model for concrete with Sea-Sand?

Hi I am running a simulation in Abaqus for a concrete beam. Its fine aggregate replaced with sea-sand. I was wondering if I could find studies that measured the ultimate strain of concrete made...

22 February 2021 6,409 2 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View