I am facing consistency issue in Hac-1 Splicing?

I am studying Unfolded protein response in yeast and I am checking Hac-1 mRNA splicing through semi RT-PCR followed by PCR. But I did not found any similar pattern of Hac-1 splicing in at least...

03 September 2020 7,346 2 View

Preventive Maintenance Database failure?

I'm currently making an investigation of how to apply RCM with Preventative Maintenance to a truck fleet of fuel transport. In my company there's not a list of functional failure or failure modes,...

29 March 2020 5,769 3 View

Has anyone attended INTED (International Technology, Education and Development) conference in Valencia? What's your experience like?

I've been invited to present in INTED2020 and would be grateful for any piece of helpful information regarding attending the conference.

02 January 2020 3,448 2 View

Why does the brain neuron not utilize glucose directly ?

Brain needs glucose as main source of energy but needs non-neuronal cells to consume the glucose and convert it to some intermediates for brain use.

15 October 2019 5,242 1 View

How is glutamate recycling coupled to energy metabolism in the CNS ?

Glutamate is a neurotransmitter in the CNS.

15 October 2019 1,439 1 View

What would be the viable Research topics in Fiscal Policy and Monetory Policy?

I need research areas worth exploring in relation to Fiscal and Monetary Policies

15 September 2019 3,849 4 View

How can I modify the surface of silver Ag (CeO2) nano-particles with -COOH for EDC/NHS reaction?

I am looking for a working protocol to modify the surface of silver NP with carboxylic acid (because I want to use EDC/ NHS crosslinkers to link my NP to antibody). I have found an article related...

06 July 2019 5,914 2 View

Can anyone help me with link of e-book or pdf version of books related to "Tea Cultivation"?

I need book related to Tea Cultivation. If anyone have any links or pdf version of books related to tea cultivation, please help me with that. Thanks in advance. Prince

21 April 2019 9,931 2 View

How to fit a Birch Murnaghan 3rd order EOS in Matlab or Mathematica or any software ?

I have the data of energy vs distance from DFT calculation

12 March 2019 2,170 1 View

How to measure the accuracy of object tracking methods?

can i have the matlab code?

03 February 2019 1,922 8 View

How to ablate oxytocin receptors in a specific brain region?

What technique would be appropriate to use if I wanted to ablate oxytocin receptors only in one specific brain region (in mice)? And what would the protocol be for such a procedure? Thank you.

29 November 2020 8,881 3 View

Does anyone know of a reliable source for primary antibodies against oxytocin and vasopressin for IHC studies in rats?

The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.

09 November 2020 833 3 View

Can a cross-over design control for regression toward the mean?

04 March 2020 3,302 3 View

Is it possible to distinguish predisposing, precipitating and maintaining factors when looking at the aetiology of mental disorders?

I'm currently looking at the aetiology of different mental disorders. I find it the basic model of aetiology (Margraf & Scheider, 2009) quite useful. It distinguishes between predisposing,...

12 January 2020 7,373 4 View

How to induce an increase in oxytocin and/or vasopressin neurons activity at early-life ?

I would like to know if anyone has a suggestion on how to induce, around postnatal day 10 (P10), an activation of OXT or AVP neurons in mice pups ? A stressor? some handling? a separation/reunion...

11 December 2019 1,344 2 View

What is the main physiological mechanism underlying the increment of the size of the readily releasable pool (RRP) in a presynaptic terminal?

The most accepted definition for RRP is " a small subset of the many vesicles in presynaptic bouton that is more readily released than other vesicles which are in the recycling and reserve pool",...

27 November 2019 9,016 4 View

What is the main physiological mechanism underlying the increment of the size of the readily releasable pool (RRP) in a presynaptic terminal?

The most accepted definition for RRP is " a small subset of the many vesicles in presynaptic bouton that is more readily released than other vesicles which are in the recycling and reserve pool",...

10 November 2019 7,460 4 View

How is glutamate recycling coupled to energy metabolism in the CNS ?

Glutamate is a neurotransmitter in the CNS.

15 October 2019 1,439 1 View

Curious about treatment approaches in psycho-therapy for people experiencing a substance use disorder in a confined environment?

Substance use disorders have been and will continue to be problematic for our society, what approaches can we utilize that will offer the greatest chance of success of abstinence and sobriety? It...

14 October 2019 9,528 1 View

How is glutamate recycling coupled to energy metabolism in the CNS ?

Glutamate is a neurotransmitter in the CNS.

09 October 2019 7,579 1 View