Any JAK-STAT deficient hematopoietic cell line known?

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H3K9Me2/3 western blots in S.pombe?

I have recently started working with heterochromatin . I am trying to quantify the H3K9Me levels but i am failing to do so. Sometimes i see faint duplex band . I do TCA Lysis and then follow up...

22 May 2024 2,071 2 View

Whether electric field and magnetic field can be oscillated in the opposite co-ordinate direction in the case of the unpolarized EMR?

In the Cartesian Corredinate commonly the direction of the electric field is towards the Y axis and magnetic field is towards the Z axis and the propagation is towards the X axis of EMR. Here, I...

25 September 2023 5,340 2 View

Is it possible to calculate PLQY for Solid Powder samples using PL ?

(Metal halide Perovskites)

14 September 2023 9,957 0 View

Is it possible to calculate PLQY for Solid Powder samples using PL ?

(Metal halide Perovskites)

14 September 2023 7,623 0 View

Why am I seeing a broad DPV peak from 0.2V to -0.2V when I use mod-GCE with 0.1M acetate buffer?

I have been trying to run a DPV experiment using modified Glassy carbon electrode as the working electrode, Pt as the counter and Ag/AgCl as the reference. Before modifying the electrode, I have...

01 August 2023 8,059 0 View

What are the methods can be performed in SPSS for validation of Logistic regression (when the model fits the data)?

I have been performed the multinomial logistic regression model in SPSS. The goodness of fit shows that the model fits the data. Based on my literature study, there are several methods can be...

30 July 2023 9,281 5 View

Whether Quadrantal Bearing and Reduced Bearing is same or different?

I was discussing with the students in my class on whole circle bearing and quadrantal bearing. They asked me that whether quadrantal bearing and reduced bearing is same or different. I had...

28 June 2023 5,875 4 View

What is the best strategy to get rid of endophytes when using woody stem as explants from plants maintained in arboretum?

I am trying to induce callus from woody plants grown in an arboretum. We have tried sterilizing explants using bleach but we are unable to get rid of funal contaminations. The explants are woody...

22 April 2023 1,704 2 View

How could a linear regression model be validated using SPSS or STATA? How to result out the consistency or accuracy of the same?

Kindly help.

24 March 2023 7,328 3 View

How to have different threshold for different images?

I am doing edge detection of microcell images directly taken form microscope. One image has 25 microwells containing the cells. I am cropping the 25 automatically and trying to do edge detection....

06 December 2022 3,933 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View