Anandita Basu

5 Questions 12 Answers 0 Followers

Questions related from Anandita Basu

Any JAK-STAT deficient hematopoietic cell line known?

Can anyone say whether there is a cell line which is JAK-STAT pathway defective and of hematopoietic origin?

23 February 2016 851 2 View

SDS-PAGE gel sticky, why?

I am facing a serious problem with SDS-PAGE, that too all of a sudden, my gel gets sticky and cannot be rmoved from the casting plate for further processing. I do take care about the requirements,...

11 January 2016 7,981 12 View

How can I label polyphenols with FITC?

Hello all I want to label polyphenols with FITC for cell treatment. Can anyone help me out with some protocol of labelling polyphenols with FITC, as I could not find any.

21 August 2015 8,576 13 View

Any suggestions on the dialysis of ox-LDL?

Can anyone provide me with the detailed procedure of how to go about with the dialysis of my LDL (L8292 SIGMA) which I oxidised by copper sulphate. It contains 5 mg of apolipoprotein as specified...

31 July 2015 8,591 7 View

How can I oxidize LDL for effective in vitro foam cell formation in THP1 cells?

Can anyone help me with a standardized protocol on oxidation of LDL? We have fetched LDL from Sigma-Aldrich. There are varied protocols related to it which has left me confused. If anyone could help.

24 March 2015 9,679 7 View