I am interested in using CRISPR system for gene knockout. Can anyone suggest with personal experience about the efficiency of kits provided by Ori gene.
They are offering pre made kits with different genes. one can choose the kit as per the gene of interest.
I will appreciate if anyone can suggest whether to using kit (it may be other than Ori gene) is more efficient over designing gRNA and then planning the experiment.
Kind regards
I suggest you take a look at the Addgene CRISPR resources. There is a lot of information on protocols, experiment design and gRNa design (see also the blog)
addgene.org/CRISPR
Blog.addgene.org crispr
The group of commercial "kit" makers, as well as researchers "designing" their own gRNA use pretty much the same limited set of tools. Basically it's the online script from the Zhang lab plus whatever libraries of gRNAs have been experimentally validated, plus (the better ones among them) some additional in-house validation.
Currently this means DIY design efforts and a number of different companies are largely going to end up with the same sequences. This may change over time, as some folks do more experimental validation that others. So that's basically what I'd look for with any company -- experimental validation as the distinguishing criterion. That's what tells you if any given gRNA sequence really works or doesn't...
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