Sheerien Rajput

12 Questions 19 Answers 0 Followers

Questions related from Sheerien Rajput

How to calculate the molarity of a solution if we accidentally mix two aliquots of solution with different molarity ?

Hi all, I have accidentally mixed 500ul of 60mM in 1000 ul of 6mM of the same solution. The 6mM solution was diluted from the stock (60mM) with serum free media. Now I don't want to discard it...

22 March 2017 1,238 3 View

Can any one suggest the recipe of Acidified Ethanol and what percent of it is acceptable in tissue culture ?

I need to dilute Melphalan in acidified ethanol. The insert says3 drops of HCL in ethanol. Is it really fine to add without measuring 3 drops. Moreover I have found that Ethanol is tolerated...

15 March 2017 635 0 View

Any idea of a good CD28 antibody?

I need a CD28 antibody preferably Mouse/Rabbit ANTI HUMAN. I want to use it with IHC or ICC. But kindly suggest the one working with Myeloma Cells. Any help would be appreciated.

20 December 2016 3,466 5 View

Any ideas for using CRISPR based gene knock out kits from Ori gene?

I am interested in using CRISPR system for gene knockout. Can anyone suggest with personal experience about the efficiency of kits provided by Ori gene. They are offering pre made kits with...

21 September 2016 8,947 2 View

Is it necessary to keep eggs in 70% Humid chamber for CAM assay?

For CAM assay, is it crucial to incubate eggs at 70% humidity. Cant we use Tissue culture incubators ?

12 April 2016 9,768 1 View

What to do if in lentiviral transduction experiments,the purity of GFP positive cells after FACS sorting is not 99-100 %?

I am working with third generation Lentiviral vector system and trying to transduce SiRNA for gene silencing. The problem I am facing is that the GFP sorted cells on FACS are showing 88-94%...

02 February 2016 8,609 5 View

Any protocol for Invasion assay in myeloma cells?

I am working with myeloma cells and wanted to know if any one has tried invasion assay in these type of cells. I need details about the dilution of matrigel and reagents used on both sides of the...

04 December 2015 3,563 4 View

Is estimation of viral titre an unavoidable step in Transduction experiments?

I have been working with 3rd generation lentiviral vectors. The problem is that the transduction efficiency is very low and GFP signal is expressed atleast after 72 hours (although the expression...

26 November 2015 782 10 View

Does anyone have any idea about EBV and its relationship if any to myeloma?

Any comments or suggestions in reference to EBV role in myeloma please

26 November 2015 1,008 2 View

Invasion Assay not working for myeloma cell lines. Could it be the pore size or chemo attractant?

I am trying Matrigel invasion assay for myeloma cell lines. I am using 8 microns membrane and coating the matrigel at 1: 3 conc. But am not getting results. I am using U266 cell line which is...

17 October 2014 10,048 3 View

What is the best method for characterization of HEK 293T Cells?

I have frozen vials of HEK 293T cells. Before using it in my experiments, I want to characterize it. Normally we can go for STR analysis but I want to do something less expensive yet standard such...

08 September 2014 6,016 3 View

Can anyone help with the expression of androgen receptor in triple negative breast carcinoma cell line?

I have transfected androgen receptor in a triple negative breast cancer cell line. Normally, the androgen is expressed in cytoplasm and after treatment with DHT it translocates to nucleus. But...

09 May 2014 3,222 3 View