11 Questions 18 Answers 0 Followers
Questions related from Sheerien Rajput
Hi all, I have accidentally mixed 500ul of 60mM in 1000 ul of 6mM of the same solution. The 6mM solution was diluted from the stock (60mM) with serum free media. Now I don't want to discard it...
22 March 2017 1,187 3 View
I need to dilute Melphalan in acidified ethanol. The insert says3 drops of HCL in ethanol. Is it really fine to add without measuring 3 drops. Moreover I have found that Ethanol is tolerated...
15 March 2017 601 0 View
I need a CD28 antibody preferably Mouse/Rabbit ANTI HUMAN. I want to use it with IHC or ICC. But kindly suggest the one working with Myeloma Cells. Any help would be appreciated.
20 December 2016 3,420 5 View
I am interested in using CRISPR system for gene knockout. Can anyone suggest with personal experience about the efficiency of kits provided by Ori gene. They are offering pre made kits with...
21 September 2016 8,905 2 View
For CAM assay, is it crucial to incubate eggs at 70% humidity. Cant we use Tissue culture incubators ?
12 April 2016 9,719 1 View
I am working with third generation Lentiviral vector system and trying to transduce SiRNA for gene silencing. The problem I am facing is that the GFP sorted cells on FACS are showing 88-94%...
02 February 2016 8,572 5 View
I am working with myeloma cells and wanted to know if any one has tried invasion assay in these type of cells. I need details about the dilution of matrigel and reagents used on both sides of the...
04 December 2015 3,520 4 View
I have been working with 3rd generation lentiviral vectors. The problem is that the transduction efficiency is very low and GFP signal is expressed atleast after 72 hours (although the expression...
26 November 2015 735 10 View
I am trying Matrigel invasion assay for myeloma cell lines. I am using 8 microns membrane and coating the matrigel at 1: 3 conc. But am not getting results. I am using U266 cell line which is...
17 October 2014 9,987 3 View
I have frozen vials of HEK 293T cells. Before using it in my experiments, I want to characterize it. Normally we can go for STR analysis but I want to do something less expensive yet standard such...
08 September 2014 5,959 3 View
I have transfected androgen receptor in a triple negative breast cancer cell line. Normally, the androgen is expressed in cytoplasm and after treatment with DHT it translocates to nucleus. But...
09 May 2014 3,180 3 View