Have been using the semi-quan API-ZYM test to see the abundantly expressed enzymes by the microbial consortium in a lab-scale Rc treating wastewater. The enzyme "Naphthol-AS-BI-phosphohydrolase" keeps popping out as one of the abundant enzymes, no matter if the feed is synthetic or real wastewater. I cannot find solid info on what this enzyme does. Any idea about that? PS: the substrate of the enzyme in the API-ZYM slot (no12) is "Naphthol-AS-BI-phosphate"
Dear Ebru,
Naphthol-AS-BI-phosphate is a standard phosphatase substrate, you may search BRENDA database for more information on the EC class 3.1.3..
http://www.brenda-enzymes.org/index.php4
Marco
Hello I am also in the same situation ultilizando sludge on soils with different sludge treatments that enzyme being of the highest active hope can also help us with more information about it. Cheers
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