How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to find Barcoding in archaea by Barcoding kit?

How can I find a barcoding in archaea variants (typically, acidophilics) e.g. protocol, research by using the PCR Barcoding Kit (SQK-PBK004), Nanopore Oxford technologies? Unfortunately, I have...

06 July 2018 8,960 0 View

How to define the optimal growth pH of Acidithiobacillus. The pH of culture was shifting during such bacteria growing.

When culturing species of Acidithiobacillus sp., pH of medium was shift during the cell growing. How did we figure out the optimal grow pH of such species. Should we just adjusted initial pH at...

09 August 2017 5,274 2 View

How to define the optimal growth pH of Acidithiobacillus. The pH of culture was shifting during such bacteria growing.

When culturing species of Acidithiobacillus sp., pH of medium was shift during the cell growing. How did we figure out the optimal grow pH of such species. Should we just adjusted initial pH at...

07 August 2017 9,816 2 View

Can we use acidophilic organisms to regenerate polluted soils?

Beside lower pH levels, acid soils, in particular soils of coniferous forests, are characterized by the presence of metals in dissociated (thereby bioavailable) cationic form (aluminum, heavy...

04 May 2016 837 1 View

How can I prepare solid media for long term storage of Acidithiobacillus ferrooxidans?

Is there any method for the long term storage of Acidithiobacillus species as we need to subculture it every 2 weeks?

15 September 2015 7,733 3 View

How can I prepare solid media for long term storage of Acidithiobacillus ferrooxidans?

Is there any method for the long term storage of Acidithiobacillus species as we need to subculture it every 2 weeks?

08 September 2015 6,956 3 View

How can we check the growth of Acidithiobacillus ferrooxidans?

whether the change in the color of media to brown is the reliable one because ferrous can also be oxidized by light? Second thing is that if the waste is also producing the same color on...

08 September 2015 1,907 5 View

I want to know if ATCC # 21834 (Acidithiobacillus Ferrooxidans bacteria) are phototactic bacteria?

I need a bacterial strain which has phototactic behavior and metal leaching behavior together. Is there any strain like this?

07 August 2015 9,661 2 View

Can you recommend a media to isolate acidophilic chromium resistant bacteria?

14 March 2015 5,977 3 View

Can you recommend a media to isolate acidophilic chromium resistant bacteria?

02 March 2015 1,967 3 View