Hello, I'm currently working on two GPCRs in insect sf9 cells and I was expecting quite low levels of expression, which I got. I am unable to find any papers that actually give a value to their level of receptor expression, usually they just say relatively high or low. Ideally I want to compare my expression levels against other GPCRs but I cannot seem to find any papers, any help would be appreciated greatly!
Thank you
It would probably need to be relative to other receptors on the same sample, or within a pipeline within the same lab. The lab next to ours worked on GPCRs and they would check the expression level with radioactive scintillation for ligand binding.
Generally one does a binding assay and determine the Bmax for specific ligand binding
I think its worth looking at the papers published by the group of Eamonn Kelly from the University of Bristol. They are interested at biased agonism at the GPCR but the quantify the expression levels as well (mostly by GTPgammaS assays)
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