Between therapy of acceptance and commitment and dialectical therapy behaves, which is better to build an intervention program to suicidal behavior?

I am interested in building a program from third generation therapies for factors associated with suicidal ideation and intent in university students

03 April 2019 7,322 1 View

Please, could you give a very short explanation of statistical methods used in species identific./reclassification by means of molecular markers?

This is a question on statistical methods used in DNA research Lately, many plant species are changing from species, genera, or even families due to studies in molecular makers. For example,...

07 September 2018 5,371 3 View

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10 July 2017 5,314 5 View

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06 July 2017 1,662 6 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

21 March 2016 4,523 8 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

02 March 2016 4,413 9 View

Which medium is the best for ATCC A2B5 hybridome culture?

24 January 2016 8,117 3 View

Which medium is the best for ATCC A2B5 hybridome culture?

31 December 2015 9,162 4 View

Can we freeze the trypan blue solution?

We wonder if the freezing may affect to the properties of the trypan blue. Thank you in advance!

11 December 2015 4,296 5 View

Any reason for the absence of policlonal activation of CD3+ T-cells in culture?

Hi everybody, I've been growing T cells from a flow cytometry CD3-sorting for a long time, obtained from C57BL6 mice spleens. At least three hours before seeding, the p96-well plate is coated...

08 June 2015 9,795 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View