Generally speaking, when used as a probe to detect the singlet oxygen, TEMP will generate three equally-intense peaks in the Electron paramagnetic resonance (EPR) spectrum, due to its conversion to TEMPO. However, the three peaks were not equal in my experiment. Any idea about the reason? In such a case, can I still give the conclusion that singlet oxygen was formed?
peak intensity changing of EPR is much complicated.
in your case, DEP (dynamic electron polarization) is also included except that mentioned by Micheal.
singlet oxygen with strong quenching ability, that why when you are doing EPR experiment of liquid sample in room temperature N2 or argon is needed to degas.
in experiment, maybe you can degas 10min first to ensure whether peaks intensity become equally. if it is, then the DEP reason is the main part. (reference could be found with Akio Kawai + singlet oxgen or RTPM)
best wishes
Mr Pach, Fu and Khudyakov, thanks for your contributions to my questions. These answers are very helpful for me to understand my situation. Best wishes.
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