Hello all,

I am wondering if there is any simple way to isolate intact (medium or high quality) total RNA from selected zoo animals. What is the best material for this? Fresh saliva, droppings, hair or something other? I am interested in some tumor supressor gene, which is probably low expressed.

My laboratory workflow:

sampling material -> conservation in RNALater -> RNA isolation -> reverse transcription into cDNA -> PCR -> sequencing PCR amplicon

Invasive sampling of whole blood or tissue is not possible due to many formalities and legislative obstruction.

Has anybody experiences with it? What is the best material and method for animal non-invasive intact RNA isolation?

Thank you very much for answers :-)