I now extract 20 gram soil by hot/cold water by 30 mins shaking and 30 mins centrifuge. One of the parameters for analysis is δ 13C. I need to freeze dry extract to get the solid matter for measuring. However, after 1st extraction, the volume of extraction seems to be too little, that's why I want to do 2nd time extraction by adding water again. Anyone has experience on this field? Many thanks.