I am growing Microalgae in wastewater and found algal lipid accumulation was increased (37.62% w/w) as compared to BG11 (29.39% w/w), and the cell size decreased.I have doubt if lipid content increased in the cell then why cell size decreased for more lipid accumulation cell need more cell volume as per my view may be I am wrong, then how it is possible cell volume will decrease and lipid content increased.
If due to stress cell volume decreases then it may be the possible reduction in cell organelles reduction and ER that store TAG (storage lipid).
Do you know if lipid increased is per cell?
The N/P ratio has an effect on cell size.
If you know the N/P of the BG11 and the waste water you are using, you could correlate the decrease in cell size with this ratio. I am guessing that the PO4 in the waste water that you are using is much higher than the standard PO4 in BG11 (in UTEX BG11 recipe N/P is very high) which could make cell size small as in presence of high P, cells will be dividing more rapidly and hence their cell size would decrease.
In addition, you can analyse the lipid profile to know which particular type of lipid is increasing in the waste water.
It will be interesting if you observe differences in the storage and membrane lipids profile under two different culture conditions. Cells could accumulate more phospholipids in waste water!
This may give some clue:
Strategies for Lipid Production Improvement in Microalgae as a Biodiesel Feedstock
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