How to select pixels by color in ImageJ?

I want to use ImageJ for counting my different tephra populations in a thin section sample, but I am not able to separate the lighter populations from the darker ones in ImageJ before counting. I...

28 September 2020 5,471 2 View

Which statistical analysis should I follow for analyzing the effects of two factors in two different cell types cultured in vitro?

Hello all! I have performed experiments involving two different cell types. These two cell types have been cultured in vitro and measurements have been performed after 4, 7 and 10 days of culture....

18 March 2020 6,337 10 View

Does anyone has any protocol to decrease cell membrane fluidity on extracellular vesicles (EVs)?

To determine the content of EVs and their possible biological impact on different cells can be challenging. Interestingly, we have found some EVs can induce differentiation on certain specific...

22 September 2019 9,776 1 View

Importance of drying-time after using gelatin as coating?

I am culturing stem cells on plates coated with gelatin. After finding so many variations of drying time in different protocols, I am wondering what is the reason? In other words, why is it...

09 September 2019 9,415 3 View

How can I create Venn diagrams from quantitative proteomics data?

Hey there! I have performed a quantitative proteomic analysis using TMT labelling and I have the results in an excel file as pairwise comparisons of every identified protein. Proteins were...

10 May 2019 1,701 11 View

Does anyone know if Allprotect Tissue Reagent (QIAGEN) is a buffered salt solution?

We are collecting human samples and thinking to use some storage buffer to ensure optimal preservation of RNA and DNA, among other molecules. We saw several papers, and authors mentioned...

09 April 2019 4,203 1 View

Bind or draw small protein with organic molecule?

Hello everyone, I want to bind or draw a proteins with an organic molecule and get them in pdb format. There is a free software to do that? Best Andrea

28 February 2019 6,279 3 View

No title

At the moment we are developing a database that contains a large number of records on this type of inventory focused on these plants. We are looking for any information presented for example in...

18 November 2018 4,881 1 View

Does anyone knows how normal is to find a total bone regeneration in a 7 year old child following a large carinectomy after 12 months follow up?

There is a 7 year olf child who suffered a traumatic head injury with a substantial brain hemorrage and a craniectomy was done to relieve pressure thus avoiding medulla herniation. A temporal...

02 March 2018 4,924 0 View

How can I calculate Langmuir adsorption isotherm parameters, as well as error in them, from experimental data?

09 October 2014 4,424 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Failed Chromatograms on HPLC-ESI Orbitrap?

Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...

09 February 2021 3,595 8 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View