Can anyone simplify the process of isolating compounds from medicinal plants after crude extracts have been prepared and phytochemical screening has been done. Any guidance will assist.
The easiest way to learn it, is to join a chemist in the laboratory and learn the bench work. Isolation of compounds from crude extracts is a process that demands a lot. Thus, can not be learn only online and going through literature alone will not help you. Join a chemist in the natural plant laboratory, observe the process and master the steps then try something under the supervision of the chemist.
Goodluck
Yes, if you are graduated from scientific department.
The process going through the following steps:
1- collection 2-washing with water 3- drying ( under shade) 4-grouding 5- extraction with different solvents( start from non-polar) 6- taking TLC 7- evaporation of solvents 8- separation (using different chromatographic techniques) 9- identification of the single pure compounds(using different spectroscopic methods, IR, NMR, MS,...etc) 10- application in different fields.
Each step has it's detail and following scientific ways.
Finally, you need to join some lectures on phytochemistry and labs.
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