What are the good Ophthalmology journals to publish for a beginner apart from the ones having exorbitant costs ?

Details about good Ophthalmology journals publishing good work without the financial burden for a young resident in a peripheral centre.

09 August 2020 5,222 7 View

Simulating Raman scattering using comsol?

I want to simulate a very basic Raman scattering experiment using comsol. I shine a light on the material, dipoles oscillate and re-radiate. Can anyone help me with the basic steps involved? I...

31 July 2019 1,036 1 View

Subcellular fractionation- why do I get smear in membrane fraction only?

We are running subcellular fractionation of transfected cells with our protein in question to determine its localization. The protein is HA tagged and we are running the fractions on a western...

13 November 2017 2,444 7 View

Which method is often used for purifying organic matter extracted from soils?

03 October 2017 2,317 7 View

Are there shared TCRs in tumor bearing C57Bl/6J mice?

I wonder wether there are shared TCRB-amino acid sequences in tumor bearing C57Bl/6J mice. We performed an immunoSEQ assay and found no overlap between our mice regarding the TCR repertoire of...

03 January 2017 5,078 2 View

Which statistical test suits best?

Hello everyone, I'm currently trying to find out which statistical test I can use to show differences between two or more populations. I had cells, which I infected with a virus +/- additional...

27 May 2016 6,675 5 View

Why has my transient transfection stopped working?

Hello I've been transfecting HeLa cells with an rtTA plasmid that contains GFP and my protein of interest. I use Xfect kit from clontech (it's polymer based. you transfect, change medium after 4h...

14 February 2016 2,446 4 View

What is energetically more favorable?

Hey, I've got a question which might be very simple. I was asked which interaction of amino acids is more energetically favorable. My problem begins with the what the definition of "energetically...

31 January 2016 2,964 6 View

How do you calculate annealing temperature for bisulfite primer?

20 January 2015 5,438 2 View

Does anyone know how to do simulation of MLL with optisystem software?

12 September 2014 8,271 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

If you haven't computer? how you show the photons to pupils?

Educational Process

01 March 2021 1,827 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Does anyone ever solved this following Matlab error : "Error using imaqdevice/start (line 95) pointgrey: Failed to stop the camera acquisition." ?

I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...

22 February 2021 6,386 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View