Any advice on triple quadrupole mass spectrometry ms/ms mode determination of protease used peptide produced?

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Need help is coverslipping-Histology samples?

Hello, Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically,...

12 July 2024 7,016 3 View

Why are bovine satellite cells differentiated at 30% confluency?

Hi, I have been culturing bovine digest satellite cells recently. In my very latest study, i seeded it at 2.65 million cell in T75 with dmem/f12+20% fbs and 1% antian and 10ng/mL fgf and tried...

24 July 2023 9,514 0 View

Can anyone tell me the sequence for CD3 antibodies: Clone SP7, Clone EPR20752, Clone 17A2, Clone 145-2C11?

I would like to find out the amino acid sequence for a few CD3 antibodies. Does anyone also know any platform/database that I can find amino acid sequence of antibodies?

20 July 2023 5,766 1 View

Cell lysates from normal human epidermal keratinocytes, adult (NHEK) for keratin filaments quantification?

Greetings fellow researchers. I am doing experimental work on normal human epidermal keratinocytes, adult (NHEK) monolayer cell culture and I am interested to analyse the proliferation and...

17 July 2023 6,270 0 View

What is limitation of electrorotation for objects with nanometer size?

Eletrorotation was used for the measurement of electrical property of cells like dielectric constant and there were also some works for the electrorotation of nanowire, but the length was still...

14 March 2023 1,489 0 View

G2/M peak is far from G0 peak in cell cycle analysis?

Hello everyone. I have performed cell cycle analysis by FCM using FxCycle PI/RNase staining solution from Invitrogen for MSCs. I fixed my cells with 70% ethanol for 2 hours at 4 degree C before...

02 February 2023 2,302 2 View

How to avoid the MHD activities during the plasma current ramp up phase in TOKAMAK?

We know the MHD such as the NTM ,Kink mode would affect the confinement or the transport of the plasma in TOKAMAK.They have different driven free energy but not only that.In general, the MHD will...

28 August 2022 3,023 1 View

What is the proper procedure to produce Fatty Acid Chloride by using Palmitic Acid and Thionyl Chloride?

I was entrusted with the project to find out the experimental procedure of producing Fatty Acid Chloride using Palmitic Acid and Thionyl Chloride, mostly the resources online are general so I need...

01 March 2022 1,927 2 View

Why is there a difference in BCA mitochondrial protein quantification results using DPBS and storage buffer?

I isolated mitochondria using Sigma-Alrich Mitochondria Isolation kit for Cultured cells (MITOISO2) and resuspended the mitochondria pellet using their Storage Buffer (50 mM HEPES, pH 7.5,...

11 February 2022 8,392 4 View

What's your thoughts on ArcGIS Pro v2.9 accuracy assessment tool?

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How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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Can you connect an HPLC to a Mass Spec only at a certain time point?

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Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

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Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

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How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

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GC-MS retention index prediticon?

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08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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08 August 2024 8,329 0 View

How are iso-frequency contours plotted?

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Sture Forsén

Bernadine - this is not my field of expertise and I have forwarded your question to Peter James - who is an MS/protomics expert.

All the best

Sture

Bernadine - Peter James quick response was that it must be trypsin since your sequence ends in an Arg!

Best'

Bernadine Ang

I knew trypsin though the reason why was a mystery at first. Thanks!