Hey all, I've been having issues with my bioanalyzer results, where the electrophoresis doesn't run all the way through the length of the "gel", and the bands are all squished down at the bottom.
I know it is not the priming station or the machine because someone else used them successfully just an hour before. The Agilent DNA kit was also new and not expired, and the gel-dye mix was made just 5 days ago.
I am wondering if any one has seen results like this?
Hi,
My first guess would be that the gel-dye mix 1) was not vortexed, 2) was not at room temperature, 3) had the wrong cc. One would also get similar results if the wrong program was started (eg starting the HS program for a 1000 kit)
Out of curiosity did you fill each sample well - even those wells with no sample? In our lab, vortexing is usually primarily the cause of failed lanes, but I find it odd that the last 4 lanes are all erroneous, which is why I asked the initial question.
The gel was at room temp and it definitely was the right program.
There are samples in only the first 8 wells. The last 4 had only water added.
I didn't vortex my gel.. that seems to be the most probable cause for my problem. Thanks for the suggestions! I will redo it and update you guys if it is the case.
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