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Questions related from Jonas Boateng
I am working with antibody microarrays printed on epoxy glass. The slides are incubated with serum followed by a secondary antibody conjugated with a fluorescent dye. 10-15% of the time we are...
07 December 2020 2,544 2 View
I am designing a PCR to detect the presence of Proteus vulgaris. Unfortunately, all the literature I have come across use either the 16S rRNA gene or the 16S-23S ITS. Does anyone know of a...
28 September 2017 5,767 1 View
I am trying to mimic a wound/pus/abscess matrix to spike in reference pathogens to test a PCR method I have developed. Unfortunately, I don't have access to any sterile pus/abscess/wound samples...
23 January 2017 5,743 2 View
I am looking for an internal control (IC) that can be spiked into clinical specimens pre-extraction devoid of any background E. coli gDNA. We currently use a plasmid containing a commercially...
21 July 2016 1,518 3 View
Our facility is performing 16S rDNA next-gen amplicon sequencing on the Ion Torrent PGM platform of mock microbial community containing reference gDNA isolates of 30 bacterial strains. The...
14 October 2015 2,458 6 View
I am performing 16S Metagenomic targeted sequencing to profile a mock bacterial community containing 27 bacteria species. My project requires the exact position or loci of the hypervariable...
02 March 2015 5,182 4 View
I have been running a series of singleplex and multiplex PCR reactions for several weeks now using E. coli genomic DNA. I typically get anywhere between 50ng/uL and 120ng/uL for 50uL and 25uL...
19 September 2014 5,289 4 View
