1. I have mapped about 20 epitopes and intend to link them using the GS linker before synthesizing and subsequent cloning and expression, am i suppose to insert the (GGGGS)3 linker between each epitope? that is what i actually did, but am not comfortable with the look of the construct. Any advise please?
2. i will also appreciate inputs on the mammalian codon pair optimization as i really do not know much about it.
Thank you