Any advice on how to use Tris Tricine SDS 16% gels (Acrylamide) in Western Blot?

Krishnajina Amarajeeva @Krishnajina-Amarajeeva

22 June 2023 0 3K Report

I have some samples with a molecular weight of 10 kDa. and with 12% gels, I cannot detect it. Therefore, I'm trying to run my samples with 16% gradient gels, and the transfer system does not work well.

I use the commercially available gels and transfer membrane sandwiches (PVDF).

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